Plasmid Preparation:Article Title: Mutations near the cleavage site of enterocin NKR-5-3B prepeptide reveal new insights into its biosynthesis.
Article Snippet: .. Bacterial strains and plasmids used in this study Strain or plasmid Description* Reference or source† Strains Enterococcus faecalis JH2-2 Plasmid-free derivative of Enterococcus faecalis JH-2; used as heterologous host for Ent53B expression [21, 47] Lactococcus lactis ATCC 19435T Bacteriocin indicator strain ATCC Listeria innocua ATCC 33090T Bacteriocin indicator strain ATCC Lactobacillus sakei subsp. sakei JCM 1157T Bacteriocin indicator strain JCM Pediococcus pentosaceus JCM 5885 Bacteriocin indicator strain JCM Bacillus coagulans JCM 2257T Bacteriocin indicator strain JCM Escherichia coli DH5a Plasmid storage strain Novagen Plasmids pNK-B1234 Cmr, pMG36c derivative containing enkB, B1234 [21] p(D 22–1) Cmr, pNK-B1234 derivative with leader moiety truncation at 22 to 1 This study p(D 22–6) Cmr, pNK-B1234 derivative with leader moiety truncation at 22 to 6 This study p(D 22–11) Cmr, pNK-B1234 derivative with leader moiety truncation at 22 to 11 This study p(D 22–16) Cmr, pNK-B1234 derivative with leader moiety truncation at 22 to 16 This study pEnt53BL-CirA Cm r, pNK-B1234 derivative with Ent53B leader moiety and circularin A propeptide This study pEnt53BL-LcyQ Cm r, pNK-B1234 derivative with Ent53B leader moiety and leucocyclicin Q propeptide This study pEnt53BL-LycQ Cm r, pNK-B1234 derivative with Ent53B leader moiety and lactocyclicin Q propeptide This study *Cmr, Chloramphenicol resistance. .. †ATCC, American Type Culture Collection, Rockville, MD USA; JCM, Japan Collection of Microorganisms, Wako, Japan. concentrations that yielded clear zones of growth inhibition on the indicator lawns.
Expressing:Article Title: Mutations near the cleavage site of enterocin NKR-5-3B prepeptide reveal new insights into its biosynthesis.
Article Snippet: .. Bacterial strains and plasmids used in this study Strain or plasmid Description* Reference or source† Strains Enterococcus faecalis JH2-2 Plasmid-free derivative of Enterococcus faecalis JH-2; used as heterologous host for Ent53B expression [21, 47] Lactococcus lactis ATCC 19435T Bacteriocin indicator strain ATCC Listeria innocua ATCC 33090T Bacteriocin indicator strain ATCC Lactobacillus sakei subsp. sakei JCM 1157T Bacteriocin indicator strain JCM Pediococcus pentosaceus JCM 5885 Bacteriocin indicator strain JCM Bacillus coagulans JCM 2257T Bacteriocin indicator strain JCM Escherichia coli DH5a Plasmid storage strain Novagen Plasmids pNK-B1234 Cmr, pMG36c derivative containing enkB, B1234 [21] p(D 22–1) Cmr, pNK-B1234 derivative with leader moiety truncation at 22 to 1 This study p(D 22–6) Cmr, pNK-B1234 derivative with leader moiety truncation at 22 to 6 This study p(D 22–11) Cmr, pNK-B1234 derivative with leader moiety truncation at 22 to 11 This study p(D 22–16) Cmr, pNK-B1234 derivative with leader moiety truncation at 22 to 16 This study pEnt53BL-CirA Cm r, pNK-B1234 derivative with Ent53B leader moiety and circularin A propeptide This study pEnt53BL-LcyQ Cm r, pNK-B1234 derivative with Ent53B leader moiety and leucocyclicin Q propeptide This study pEnt53BL-LycQ Cm r, pNK-B1234 derivative with Ent53B leader moiety and lactocyclicin Q propeptide This study *Cmr, Chloramphenicol resistance. .. †ATCC, American Type Culture Collection, Rockville, MD USA; JCM, Japan Collection of Microorganisms, Wako, Japan. concentrations that yielded clear zones of growth inhibition on the indicator lawns.
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